What is the three-dimensional culture microplate "TASCL"?
Human iPS cells cultured on the three-dimensional culture microplate TASCL
TASCL (Tapered Stencil for Cluster Culture) is a cell cluster three-dimensional culture microplate developed based on university-developed microfabrication and surface treatment technologies. Its features include the ability to mass-culture cell clusters (spheroids and embryoid bodies) of almost uniform shape and size at once, the ability to induce differentiation using the same equipment through long-term culture for about one month, the ease of use including medium replacement, the ability to observe cell clusters on TASCL under a microscope during culture, and the high culture density that keeps the costs of medium and reagents low.
Package of the three-dimensional culture microplate TASCL
The TASCL product package is a six-hole plate with six culture inserts installed, and a silicone microwell plate installed on top of the porous membrane of the culture insert. The microwells and culture inserts are coated with a cell adhesion inhibitory coating (valid for 2 years from manufacture), and each set of 6-well plates is packaged in a bag and treated with gamma rays (25 kGy to 50 kGy). Therefore, it is expected that you will use all 6 microwell plates at once after opening the bag.
The three-dimensional culture microplate TASCL can currently be used for research purposes. Cell clusters cultured in TASCL are not permitted to be administered to humans, except for clinical research. Please contact us regarding clinical research.
The bottom of the microwell part of TASCL has holes, and gas and culture medium are diffused through the porous membrane of the culture insert, improving the survival rate of cell clusters and spheroids.
Therefore, long-term culture of about one month is possible, and differentiation induction can be performed on the same equipment.
Eight features of cell cluster/spheroid 3D culture microplate TASCL
1) A large amount of cell clusters of almost uniform shape, size, and quality can be cultured in 3D at once
Approx. 3600 cells in TASCL 600 wells, and approximately 6000 cells in TASCL 1000 wells
Please note that the quality standard takes into account that cell clusters cannot form in up to 5% of the microwells.
2) The condition of cell clusters and spheroids can be maintained for a long time
Because the bottom is perforated and gas and medium circulate
3) The same equipment can be used for everything from cell cluster culture to differentiation induction
Because gas and medium circulate, long-term culture can be performed for up to one month
4) The cost of medium and reagents can be reduced
Due to the high-density well structure, less medium and reagents are used
5) Ready to use after opening
Coated to inhibit cell adhesion (expiration date is two years from date of manufacture)
Irradiated with 25kGy to 50kGy gamma rays
6) Easy to use, such as easy medium replacement
Simply sow the cell suspension from above to seed (no centrifugation required)
Easy to change the medium because the cell masses are not touched when changing the medium (no chance of spilling the cell masses)
7) Easy to observe the cell masses on TASCL even while culturing
Microscopic observation of cells is possible while they are on TASCL
8) Easy to culture co-cultured cell masses in 3D
Easy to form cell masses by co-culturing 2 or 3 types of cells
Features of the TASCL cell cluster 3D culture microplate
In regenerative medicine, there are various benefits to culturing cells in 3D to use them as cell clusters and spheroids.
Single cells vs. cell aggregates
Methods for 3D culture of cell clusters include the hanging drop method, the method using non-adhesive cultureware, and suspension rotation culture.
However, there were problems such as the complexity of the method, the difficulty of changing the medium, and the heterogeneity of the size, shape, and quality of the cell clusters. In other words, it was difficult to 3D culture a large number of cell clusters and spheroids of almost uniform size, shape, and quality.
| Method | TASCL | Hanging drop method |
Culture using scaffolds |
Suspension rotation culture |
Microplates other than TASCL |
| Ease of cultivation | Good | Neutral | Good | Good | Neutral/Good |
| Number of cell aggregates that can be cultivated at one time | Good | Neutral | Good | Good | Neutral/Good |
| Cultivation period | Good | Neutral | Neutral/Good | Neutral | Neutral |
| Uniformity of size and shape of cell aggregates | Good | Neutral | Neutral/Good | Neutral | Neutral |
| Quality of cell aggregates | Good | Neutral | Neutral/Good | Neutral | Neutral/Good |
| Possibility of observation during cultivation | GOod | Neutral | Neutral/Good | Neutral | Good |
Comparison of cell aggregate culture methods
TASCL is a cell cluster and spheroid 3D culture microplate developed to solve this problem. By simply sprinkling a suspension containing cells from above, you can three-dimensionally culture and induce differentiation of approximately 1000 or 600 cell clusters of almost uniform size and shape with one TASCL main unit (approximately 6000 or 3600 with one set of 6-well plates).
TASCL can three-dimensionally culture approximately 6000 or 3600 spherical cell clusters (spheroids) of almost uniform size at one time.
This uniformity is not found in other microwells.
| TASCL | Microplate A | Microplate B | Microplate C | |
| Ease of medium replacement | Good | Not Good | Not Good | Neutral |
| Long-term culture | Good | Not Good | Not Good | Neutral |
| Uniformity of cell aggregate shape and size | Good | Good | Neutral | Neutral |
Comparison of TASCL with other microplates
Size distribution of cell clusters cultured in TASCL
TASCL has two products with different microwell sizes: TASCL1000 well and TASCL600 well.
TASCL1000 is suitable for culturing cell clusters with diameters of 150 μm to 200 μm. Since there are 1020 wells in one TASCL main unit, one set of 6-well plates with six TASCL main units can three-dimensionally culture approximately 6000 cell clusters and spheroids.
The TASCL600 is suitable for culturing cell clusters with diameters of 200μm to 300μm. Since one TASCL main unit has 621 wells, one set of 6-well plates with six TASCL main units can culture approximately 3,600 cell clusters/spheroids in three dimensions.
TASCL specifications
| Product | TASCL 1000 well | TASCL 600 well |
| Model number | TASCL1000 | TASCL600 |
| Set contents | 1 6-well plate 6 culture inserts 6 TASCL1000 wells |
1 6-well plate 6 culture inserts 6 TASCL600 wells |
| Size | 23.5mm diameter circle | 23.5mm diameter circle |
| Number of micro wells | 1020 | 621 |
| Well size | Well top 500μm×500μm Well bottom 250μm×250μm Well height 500μm |
Well top 650μm×650μm |
| Estimated number of cultured cells per well | 500 to 3,000 | 2,000 to 10,000 pieces |
| Expiration date (Expiration date of cell adhesion inhibitor coating agent) |
24 months from manufacture | 24 months from manufacture |
| Gamma ray irradiation before shipping | 25kGy | 25kGy |
Recommendation comment for cell cluster three-dimensional culture microplate TASCL
TASCL is useful for cell cluster transplantation research
Professor Emeritus of Nagoya University, Director of Rheumatology and Artificial Joint Center, Nagoya Kyoritsu Hospital
Dr. Hisashi Iwata
As an example of regenerative medicine in the field of orthopedics, cell transplantation therapy for defective areas such as cartilage is being researched. Therefore, it is expected that transplanting cell clusters instead of cells will increase the engraftment rate and improve the therapeutic effect. TASCL is useful for research on such transplantation therapy because it can culture homogeneous cell clusters in large quantities on a small plate.
TASCL is easier to use than conventional products and has almost no risk of failure
Aichi Medical University, Orthopedic Surgery Professor (as of 2021)
(Currently Professor Emeritus of Aichi Medical University, Vice Director of Hiroshima Municipal Hospital)
Dr. Masataka Deka
Our laboratory is working on preclinical research into cell cluster transplantation. We used TASCL to culture cell clusters, and even staff who had never used it before were able to easily culture the spherical cell clusters.
Compared to conventional products, it is very easy to use and has almost no risk of failure. It can be said to be a useful tool for experiments using cell clusters.
Three-dimensional culture and TASCL
Cell culture was initially performed in a flat (two-dimensional) environment. Then, around the 1970s, three-dimensional culture began to be used to create an environment closer to the living body or to regenerate tissues and organs. Cell clusters (spheroids) produced by three-dimensional culture enable intercellular communication through cell-to-cell junctions, and signals between cells and extracellular matrices can also be reproduced. Experiments performed with three-dimensional cell clusters (spheroids) are thought to more accurately reproduce the in vivo environment. Three-dimensional culture can be divided into "scaffold-type culture" and "scaffold-free culture".
Scaffold-type culture allows cells to attach to scaffolds such as collagen, polymer hydrogel, and polystyrene, and then grow and differentiate. Compared to scaffold-free culture, it uses scaffolds, which are impurities for the body, so it can be said that there is a relatively high risk of cell impact and contamination.
Scaffold-free culture cultures cell clusters (spheroids) without a scaffold for the cells. There are cultures using bioreactors and cultures using microplates. Cultures using bioreactors have variations in the shape and size of cell clusters (spheroids).
TASCL corresponds to cultures using microplates, which are suspension cultures. Although the cells come into contact with the surface of the culture insert or TASCL, they do not adhere to these surfaces because they are coated with a cell adhesion inhibitor.
FAQs for TASCL
Q. How do I recover cell clusters (spheroids) that have been three-dimensionally cultured with TASCL?
A. Either strongly suck up the cell clusters together with the culture medium with a pipette, or peel the TASCL off the culture insert with tweezers and wash off the cell clusters with the culture medium to recover them together with the culture medium.
Q. What types of cells can be three-dimensionally cultured with TASCL?
A. Any type of adhesive cell commonly used in regenerative medicine research can be cultured.
As an example, we have a track record with the following cells:
・ hiPS cells (multiple strains)
・ hiPS-derived cardiomyocytes (normal, diseased)
・ HepG2 cells
・ MIN6 cells
・ Human stem cells
・ Human knee chondrocytes
・ Mouse ES cells (multiple strains)
・ Mouse primary hepatocytes
・ Mouse primary cardiomyocytes
Please note that TASCL is not suitable for culturing floating cells.
Q. Can cell clusters on TASCL be observed under a microscope?
A. TASCL microwells, 6-well plates, and culture inserts are optically transparent, so they can be observed under an inverted microscope as is.
Because a working distance of about 3 mm must be maintained between the objective lens and the insert, if the objective lens is 4x to 20x that of a typical inverted microscope, they can be observed under TASCL as is.
Q. Can cell clusters on TASCL be fluorescently stained? How can I stain them?
A. Yes, they can be fluorescently stained.
You can follow the protocol in the manual for each staining reagent,
but please note the following points.
When staining on TASCL, it is important to always operate the culture insert so that the reagent flows from the top to the bottom (front to back).
One simple method is to remove the TASCL/culture insert from the well plate, place it on a sterile drape, etc., and replace the liquid in the TASCL with the desired reagent.
Adjust the drip speed so that the liquid level does not exceed the top surface of the TASCL at any time.
If you need to wait with the reagent in it for fixation, staining, etc., remove the culture insert from the drape and leave it suspended in the air. The liquid will be held in place by surface tension.
It is also a good idea to add quantum dots or other fluorescent reagents for live imaging to the medium before seeding or during EB culture.
It is also possible to perform immunostaining and special staining used in 2D culture on TASCL.
Note that quantitative staining is not possible even if cell clusters are stained as spheres.
If quantitative discussion is required, such as comparing the degree of staining,
it is still necessary to fix and section the cells and then stain them.
In that case, it is convenient to use a gel for embedding cell clusters.
Q. I would like to perform an ELISA test on cells cultured in 3D on TASCL and cells cultured in 2D medium. Are there any differences in the technique compared to 2D medium?
A. There is no particular difference in the technique.
Q. What are the intended uses for TASCL1000 wells and TASCL600 wells?
A. The TASCL1000 wells are intended to culture 500 to 3,000 cells per well, and are intended for use in three-dimensional culture and differentiation induction of various cells in regenerative medicine research.
The TASCL600 wells are intended to culture 2,000 to 10,000 cells per well, and are suitable for the formation of three-dimensional spheroids and organoids, as they can culture larger cell clusters than the TASCL1000 wells.
Q. How do you sterilize TASCL?
A. Before shipping, they are irradiated with gamma rays at 25 kGy to 50 kGy while packaged in bags and cardboard boxes.
Q. What is the approximate seeding amount of cell suspension? Also, are there any precautions to take when seeding?
A. The standard amount of cell suspension is 500uL, but it is okay to increase or decrease the amount slightly.
Other precautions to take are to seed the cell suspension (500uL) inside the insert, wait for the cells to settle, and then add medium to the outside of the insert (well part) until the liquid level is about the same as the top surface of the TASCL.
Q. Is there a good way to detach the cell clumps from the TASCL?
A. There are the following methods.
a) If the cell clumps are attached to the TASCL side, hold the TASCL with tweezers or the like and detach it from the insert, then drop it using a water stream from a pipette into a 6-well plate or centrifuge tube containing a buffer solution or the like.
b) If the cell clumps are attached to the membrane side of the insert, hold the TASCL with tweezers or the like and detach it from the insert, and scrape off the cell clumps remaining on the surface of the insert with a scraper or the like.
Q. What is the crescent-shaped depression used for?
Also, when seeding the cell suspension, will cells enter this hole and become impurities that will contaminate the microwell?
A. This depression is designed to allow additional culture medium to be injected or aspirated after the cell suspension has been dripped.
If culture medium is injected or aspirated from above the microwell after the cell suspension has been dripped, the cells may come out of the microwell, so the amount of culture medium is adjusted in this depression.
There is a wall between this depression and the microwell, so even if impurities remain in the depression, they will not flow into the microwell as long as it is kept horizontal.
Q. The cell clumps are on the membrane (permeation membrane) of the upper culture insert, but will proteins and enzymes released from the cell clumps pass through to the lower level?
A. The size of protein molecules is about 10 nm,
and the size of enzymes is about 20 nm,
so they pass through the culture insert (PET membrane pore size 3.0 μm).
Q. Cell clumps form on the membrane of the culture insert,
but what happens to the cells that do not form cell clumps?
A. If cell clump formation is successful,
the number of small cells around them that do not form cell clumps
will be a few percent of the number of cells that make up the cell clump. In general experiments, this is a negligible level.
If the experimental system is one that detects minute differences that cannot be ignored,
after cell clump formation, we would like you to take them out into a separate container and analyze them.
Q. Please tell me the structure and size of TASCL and the size of the microwell.
A. As follows.
1) TASCL 600 well
One 6-well plate, 6 TASCLs, 6 culture inserts.
Microwell: Top 650 μm x 650 μm. Bottom 400μm x 400μm.
Height 500μm. Number of microwells 1,020.
Suitable for culturing cell clusters of approximately 150μm to 200μm.
2) TASCL 1,000 Well
6 TASCLs and 6 culture inserts in one 6-well plate.
Microwells: Top 500μm x 500μm. Bottom 250μm x 250μm.
Height 500μm. Number of microwells 621.
Suitable for culturing cell clusters of approximately 200μm to 300μm.
Q. Please tell me how to purchase TASCL (including minimum purchase quantity) and payment method.
A. If you would like to purchase TASCL, please contact our customer service.
The minimum purchase quantity is one set for both 600-well and 1,000-well plates (6-well, 6-culture insert, TASCL/set).
Your sales agent or our company will inform you of the payment method.
Q. If I order TASCL, how long will it take to arrive?
A. If you are ordering from a sales agent, please check with the sales agent.
We will ship within one week of your order (in Japan).
If you purchase directly from us, we will ship within one week of your order (in Japan).
If we are out of stock, we will contact you again.
Q. What is the expiration date of TASCL?
A. The expiration date of TASCL is two years from the date of manufacture.
This is because the expiration date of the cell adhesion inhibitor coating treatment agent is two years.
(Previously, it was one year from the date of manufacture, but this has been extended to two years. September 1, 2022)
Q. What are the advantages of TASCL compared to other companies' cell culture microplates and 3D culture equipment?
A. TASCL has the following advantages due to the perforated bottom of the microwells and its precise, uniform structure.
1) By simply dripping the cell suspension from above, you can culture approximately 3,600 cell clumps (for 600 wells) or approximately 6,000 cell clumps (for 1,000 wells) of uniform size, shape, and quality at one time.
Centrifugation is not required for cell suspension.
2) It is easy to use, and even beginners to cell culture can easily culture cell clumps of uniform size, shape, and quality.
3) The bottom of TASCL is porous, allowing gas and culture medium to circulate.
As a result, long-term culture of more than one month is possible, and differentiation induction is also possible.
4) TASCL can be peeled off from the culture insert by pulling it with tweezers, so it can be used in combination with any culture equipment.
5) Since the well depth is greater than the diameter of the cell cluster, there is almost no loss of cellularity when changing the culture medium. (Cell clusters are prevented from spilling out).
6) With approximately 600 or 1000 wells per 1.65 cm, the density is high, leading to reduced costs for culture medium and reagents.
7) Sterilized and coated to inhibit cell adhesion, the container can be used immediately after opening. However, if many air bubbles are present, they will need to be removed.
8) Cell clusters can be observed under a microscope while still on the TASCL. TASCL, culture inserts, and 6-well plates are optically transparent, so cells can be observed under a microscope while still on the TASCL.
Q. Can cell clusters cultured in 3D with TASCL be administered to humans?
A. Currently, TASCL is sold for testing and research purposes,
so administration of cell masses cultured with TASCL to humans is not permitted, with the exception of clinical research.
However, as an exception, under the Act on the Safety of Regenerative Medicine, there are cases where clinical research is being conducted in which cell masses cultured in three dimensions with TASCL are administered to humans, after going through the prescribed procedures.
Please contact us regarding clinical research.
Research results and patents on TASCL
and cell aggregate three-dimensional culture
Conference presentations
These are the results of conference presentations on TASCL, three-dimensional culture, cell aggregates, cell culture and cell processing technology, and cell transplantation, in which our company's executives and employees participated.
- 「細胞塊培養デバイスTASCLと機械学習を利用した量産細胞塊の品質予測」発表学会：2019/11/8～9、第46回日本臓器保存生物医学会学術集会、発表者：須田修矢、青山千裕、池内真志
- 「新規再生医療デバイス TASCL（タスクル）を用いた細胞塊治療 ―基礎から臨床へ―」 発表学会：2019/3/23 第18回日本再生医療学会総会 共催学術セミナー、発表者：池内真志
- 「独自開発した胚葉体培養デバイスTASCLを用いた幹細胞クラスター長期培養法と分化誘導法の開発」 発表学会：2018/3/21-23 第17回日本再生医療学会総会（パシフィコ横浜）
- 「胚様体大量培養デバイス TASCL（Tapered Stencil for Cluster Culture）を用いた幹細胞クラスター長期培養法」 発表学会：2017/11/10 第44回 日本臓器保存 生物医学会 学会集会（大阪大学中之島センター）
- 「３次元培養デバイスTASCLを用いた胚様体形成プロセスの評価」 発表学会：2017/3/7-9 第16回日本再生医療学会（仙台）
- 「胚様体大量培養デバイスTASCLを用いた幹細胞クラスターの長期間培養法の検討」 発表学会：2017/3/7-9 第16回日本再生医療学会（仙台）
- 「胚様体大量生産のための新規エラストマー培養アレイの開発」 研究者：池内真志、木部龍太、豊田悠司、生田幸士、林衆治 発表学会 2013/3/21-23 第12回日本再生医療学会総会（横浜）
- 「細胞への多糖磁性粒子複合体の付着・取り込みとTEM評価解析」 研究者：宮本義孝、斉藤弘明、野口洋文、村瀬勝俊、林 衆治 発表学会：2012/11/16-17 第39回日本臓器保存生物医学会学術集会（福島）
- 「細胞パターニングデバイスによる初代肝細胞スフェロイドの構築」 研究者：宮本義孝、池内真志、湯川博、鈴木聡、岩田久、生田幸士、林 衆治 発表学会：2011/3/1-2 第10回日本再生医療学会総会（東京）
- 「Quantum dots based imaging of adipose-derived stem cells for srem cell therapy.」
研究者：Wartanabe M, Yukawa H, Kagami Y, Kaji N, Okamoto Y, Miyamoto Y, Tokeshi M, Hayashi S, Baba Y 発表学会：2010/12/15-20 2010 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010), - 「膵島再生に向けたコンビナトリアル３次元培養デバイスの開発」 研究者：池内真志、大石幸一、野口洋文、宮本義孝、林 衆治、生田幸士 発表学会：2010/11/19-20 第37回日本臓器保存生物医学会（新潟）
Research papers
These are research results on TASCL, three-dimensional culture, cell aggregates, cell culture and processing technology, cell transplantation, and cell transplantation in which our company's executives and employees participated.
- Yoshitaka Miyamoto, Yumie Koshidaka, Katsutoshi Murase, Shoichiro Kanno, Hirofumi Noguchi, Kenji Miyado, Takeshi Ikeya, Satoshi Suzuki, Tohru Yagi, Naozumi Teramoto and Shuji Hayashi, "Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles", Materials, Nov. 2022, doi: 10.3390/ma15217823
- 江崎 ゆり子, 須田 修矢, 池内 真志「高密度培養デバイスと3次元CNNを用いたhiPS胚様体の生産効率化」, 生体医工学, 2021年 Annual59 巻 Abstract 号 325, 2021/10/17, https://doi.org/10.11239/jsmbe.Annual59.325
- 野口 洋文, 池内 真志, 潮平 知佳,「TASCLを用いた人工膵幹細胞からインスリン分泌細胞への分化誘導法の開発」, 科研費基盤(C)実施状況報告書, 2021/3/31, https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K09051/
- 河野 菜摘子, 中林 一彦, 池内 真志, 宮本 義孝,「凍結細胞の運命：バイオインフォマティクスに基づく医療用細胞の品質評価技術の構築」, 科研費基盤(B)研究成果報告書, 2021/3/31, https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18H03556/18H03556seika/
- 青山 千裕, 池内 真志「均質な細胞塊を包埋したアルギン酸ゲルシートの作成」,生体医工学, 2019年 Annual57 巻 Abstract 号 S80_1, 2019/12/27, https://doi.org/10.11239/jsmbe.Annual57.S80_1
- 須田修矢,青山千裕, 池内真志「細胞塊培養デバイスTASCLと機械学習を利用した量産細胞塊の品質予測」Organ Biology26 (3)106-106, 2019.
- 宮本 義孝, 池内 真志, 河野 菜摘子「二次元培養から三次元培養への潮流～細胞培養技術の変遷～」Organ Biology 27(1), 37-52, 2019 一般社団法人 日本臓器保存生物医学会 https://www.jstage.jst.go.jp/article/organbio/27/1/27_37/_article/-char/ja/
- Yoshitaka Miyamoto, Ph.D., Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, Shuji Hayashi, "Enhanced Adipogenic Differentiation of Human Adipose-Derived Stem Cells in an in vitro Microenvironment: The Preparation of Adipose-Like Microtissues Using a Three-Dimensional Culture", Cell Medicine, Jan. 2017, doi:https://doi.org/10.3727/215517916X693096
- Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, and Shuji Hayashi, Cell Medicine, Vol. 8, pp. 4756, 2015, "Spheroid Formation and Evaluation of Hepatic Cells in a Three-Dimensional Culture Device"http://dx.doi.org/10.3727/215517915X689056
- 宮本 義孝, 池内 真志, 野口 洋文, 鈴木 聡, 八木 透, 生田 幸士, 林 衆治「培養デバイスTASCLによる初代肝細胞スフェロイド培養、および均一・大量生産系の確立」生体医工学/53 巻 (2015) Supplement号、https://doi.org/10.11239/jsmbe.53.S230_02
- 池内 真志, 豊田 悠司, 林 衆治, 生田 幸士、「気液透過性を付与したTASCLによるhiPS胚様体の高効率生産」生体医工学/53 巻 (2015) Supplement 号 https://doi.org/10.11239/jsmbe.53.S236_01
- Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, Shuji Hayashi, "Three-Dimensional In Vitro Hepatic Constructs Formed Using Combinatorial Tapered Stencil for Cluster Culture (TASCL) Device", Cell Med, 2014 Dec 12;7(2):67-74. doi:10.3727/215517914X685187.
- Oishi K, Miyamoto Y, Saito H, Murase K, Ono K, Sawada M, Watanabe M, Noguchi Y, Fujiwara T, Hayashi S, Noguchi H. In vivo imaging of transplanted islets labeled with a novel cationic nanoparticle. PLoS One. 2013;8(2):e57046. 2013
- Yukawa H, Watanabe M, Kaji N, Okamoto Y, Tokeshi M, Miyamoto Y, Noguchi H, Baba Y, Hayashi S. Monitoring transplanted adipose tissue-derived stem cells combined with heparin in the liver by fluorescence imaging using quantum dots. Biomaterials 33(7), 2177-2186. 2012
- Miyamoto Y, Oishi K, Yukawa H, Noguchi H, Sasaki M, Iwata H, Hayashi S. Cryopreservation of human adipose tissue-derived stem/progenitor cells using the silk protein sericin. Cell Transplant. 21(2-3), 617-622. 2012
- Hayashi T, Misawa H, Nakahara H, Noguchi H, Yoshida A, Kobayashi N, Tanaka M, Ozaki T. Transplantation of osteogenically differentiated mouse iPS cells for bone repair. Cell Transplant. 21(2-3), 591-600. 2012
- Naziruddin B, Matsumoto S, Noguchi H, Takita M, Shimoda M, Fujita Y, Chujo D, Tate C, Onaca N, Lamont J, Kobayashi N, Levy MF. Improved pancreatic islet isolation outcome in autologous transplantation for chronic pancreatitis. Cell Transplant. 21(2-3), 553-558. 2012
- Takita M, Matsumoto S, Noguchi H, Shimoda M, Ikemoto T, Chujo D, Tamura Y, Olsen GS, Naziruddin B, Purcell K, Onaca N, Levy MF. Adverse events in clinical islet transplantation: one institutional experience. Cell Transplant. 21(2-3), 547-551. 2012
- Noguchi H, Naziruddin B, Jackson A, Shimoda M, Ikemoto T, Fujita Y, Chujo D, Takita M, Peng H, Sugimoto K, Itoh T, Kobayashi N, Onaca N, Levy MF, Matsumoto S. Fresh islets are more effective for islet transplantation than cultured islets. Cell Tranaplant. 21(2-3), 517-523. 2012
- Noguchi H, Naziruddin B, Jackson A, Shimoda M, Fujita Y, Chujo D, Takita M, Peng H, Sugimoto K, Itoh T, Kobayashi N, Ueda M, Okitsu T, Iwanaga Y, Nagata H, Liu X, Kamiya H, Onaca N, Levy MF, Matsumoto S. Comparison of ulinastatin, gabexate mesilate, and nafamostat mesilate in preservation solution for islet isolation. Cell Transplant. 21(2-3), 509-516. 2012
- Shimoda M, Noguchi H, Fujita Y, Takita M, Ikemoto T, Chujo D, Naziruddin B, Levy MF, Kobayashi N, Grayburn PA, Matsumoto S. Islet purification method using large bottles effectively achieves high islet yield from pig pancreas. Cell Transplant. 21(2-3), 501-508. 2012
- Noguchi H, Naziruddin B, Shimoda M, Fujita Y, Chujo D, Takita M, Peng H, Sugimoto K, Itoh T, Kobayashi N, Onaca N, Levy MF, Matsumoto S. Evaluation of osmolality of density gradient for human islet purification. Cell Transplant. 21(2-3), 493-500. 2012
- Shimoda M, Noguchi H, Fujita Y, Takita M, Ikemoto T, Chujo D, Naziruddin B, Levy MF, Kobayashi N, Grayburn PA, Matsumoto S. Improvement of porcine islet isolation by inhibition of trypsin activity during pancreas preservation and digestion using α1-antitrypsin. Cell Transplant. 21(2-3), 465-471. 2012
- Takita M, Matsumoto S, Qin H, Noguchi H, Shimoda M, Chujo D, Itoh T, Sugimoto K, Onaca N, Naziruddin B, Levy MF. Secretory unit of islet transplant objects (SUITO) index can predict severity of hypoglycemic episodes in clinical islet cell transplantation. Cell Transplant. 21(1), 91-98. 2012
- Takasaki Y, Watanabe M, Yukawa H, Sabarudin A, Inagaki K, Kaji N, Okamoto Y, Tokeshi M, Miyamoto Y, Noguchi H, Umemura T, Hayashi S, Baba Y, Haraguchi H. "Estimation of the distribution of intravenously injected adipose tissue-derived stem cells labeled with quantum dots in mice organ through the determination of their metallic components by ICPMS" Anal. Chem. 83(21), 8252-8258. 2011
- Noguchi H. Pancreas procurement and preservation for islet transplantation: personal considerations. J. Transplant. 2011, 783168
- Ochiai K, Watanabe M, Ueki H, Huang P, Fujii Y, Nasu Y, Noguchi H, Hirata T, Sakaguchi M, Huh NH, Kashiwakura Y, Kaku H, Kumon H. Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1. Biochem. Biophys. Res. Commun. 412(2), 391-395. 2011
- Takita M, Matsumoto S, Noguchi H, Shimoda M, Chujo D, Itoh T, Sugimoto K, Sorelle JA, Onaca N, Naziruddin B, Levy MF. Cluster analysis of self-monitoring blood glucose assessments in clinical islet cell transplantation for type 1 diabetes. Diabetes Care 34(8), 1799-1803. 2011
- Yukawa H, Noguchi H, Hayashi S. "Embryonic body formation using the tapered soft stencil for cluster culture device. " Biomaterials 32(15), 3729-3738. 2011
- Yukawa H., Kagami Y., Watanabe M., Oishi K., Miyamoto Y., Okamoto Y., Tokeshi M., Kaji N., Noguchi H., Ono K., Sawada M., Baba Y., Hamajima N., Hayashi S. Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells. Biomaterials 31(14), 4094-4103. 2010
- Yukawa H., Noguchi H., Oishi K., Takagi S., Miyamoto Y., Hayashi S. Cell Transplantation of Adipose Tissue-Derived Stem Cells in Combination with Heparin Attenuated Acute Liver Failure in Mice. Cell Transplant. 18(5-6), 611-618. 2009
Patents
The status of patents and applications related to TASCL is as follows:
1. Nin'i no bunpu keijō to bunpu mitsudo o yūsuru bunshi matawa ryūshi no shūdan o dōjini tashu tairyō seisei suru hōhō to sono hōhō ni shiyō suru masuku-zai shutsugan bangō: Toku-gan 2008 - 237696 (P 2008 - 237696) shutsugan-bi: 2008-Nen 9 tsuki 17-nichi kōkai bangō: Tokkai 2010 - 68728 (P 2010 - 68728 A) kokusai kōkai bangō: WO 2010/ 032595 A 1 shutsugan hito: Dokuritsugyōsei hōjin kagaku gijutsu shinkō kikō hatsumei-sha: Ikuta Kōshi, Ikeuchi Masashi 2. Saibō baiyō-yō shītooyobi sono seizō hōhō shutsugan bangō: Toku-gan 2014 - 123483 (P 2014 - 123483) shutsugan-bi: 2014-Nen 6 tsuki 16-nichi kōkai bangō: Tokkai 2016 - 2023 (P 2016 - 2023 A) shutsugan hito: Nittōdenkō kabushikigaisha, Ikeuchi Masashi, ippan zaidanhōjin gurōbaruherusukea zaidan hatsumei-sha: Toyota Yūji, Ikeuchi Masashi, Hayashi Shū Osamu 3. Saibō baiyō sōchi oyobi saibō baiyō hōhō shutsugan bangō: Toku-gan 2014 - 232224 (P 2014 - 232224) shutsugan-bi: 2014-Nen 11 tsuki 14-nichi kōkai bangō: Tokkai 2016 - 93149 (P 2016 - 93149 A) shutsugan hito: Ikeuchi Masashi hatsumei-sha: Ikeuchi Masashi, Hayashi Shū Osamu, Toyota Yūji
